Coding
Part:BBa_K3204002:Design
Designed by: Felix Schweigkofler Group: iGEM19_NAWI_Graz (2019-10-16)
Receptor binding protein of paenibacillus phage HB10c2
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 649
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Three internal PstI sites have been removed, without altering the amino acid sequence.
Source
The source of this part is the paenibacillus phage HB10c2. The gene has been codon optimized for the expression in E. coli with the tool from Thermo scientific